A globulin-1 regulatory region is shown, a nucleotide sequence of
approximately 3 kb which provides improved seed preferred, and
particularly embryo preferred expression in plants. Methods of use are
also shown in preferentially expressing a heterologous protein to the
embryo tissue of a plant. The sequence is particularly useful in
expression of heterologous proteins to the embryo of monocotyledonous
plants, particularly cereals, and maize.