The invention provides primers designed on base of the difference of
sequences among staphylococcal enterotoxin subtypes, i.e., C.sub.1,
C.sub.2 and C.sub.3. The invention also provides a PCR method for
detecting subtypes of staphylococcal enterotoxin type C by using the
above-mentioned primers. The invention relates also to DNA probes useful
for the identification of subtypes C.sub.1, C.sub.2 and C.sub.3 of
staphylococcal enterotoxin type C in various food and clinical samples.
The primers of the invention comprises following sequences:
ENTC1 5'-ACAGA GTTAT TAAAT GAAGG-3';
ENTC2 5'-GTATC AGCAA CTAAA GTTAT-3';
ENTC3 5'-AAGAG ATTAT TTATT TCACGT-3';
ENTCR 5'-ATCAT ACCAA AAAGT ATTGC-3'.