The present invention herein discloses a method for purifying human serum
albumin, which is characterized in that it comprises the steps of
heat-treating a human serum albumin-containing solution including
impurities originated from a host cell, in the presence of a divalent
cation such as calcium ion, magnesium ion, nickel ion, cobalt ion, iron
ion and zinc ion to thus selectively allow the impurities to undergo
agglutination. The present invention also provides the highly purified
human serum albumin prepared by the foregoing method.